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Researcher || SciCommer- My Lab Diary on Instagram: "1X Phosphate Buffer Saline (PBS) Recipe(1L) 137mM NaCl=8.00g 2.7mM KCl=0.20g 10mM Na2HPO4.2H2O=1.78g 1.8mM KH2PO4=0.24g Recipe credit: @merckgroup -Take 800ml deionized water -Add the above mentioned amount -Adjust pH at 7.4 using 1M/1N HCl -After adjusting pH add rest of the water to make it 1L ✨Look carefully whether you are using dihydrate or heptahydrate of Na2HPO4. As the 2 compounds have a different Molecular Weight, thus the amount will
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