Western blotting allows scientists to identify specific proteins ... before and during immunoblotting helps prevent nonspecific antibody binding on the membrane. 6 The primary antibody, specific to ...

Directly-labeled primary antibodies also allow researchers to multiplex using ... Never assume that antibodies validated for one application, such as western blotting, will work well in another, such ...

The FASTA files from Stage B are annotated using a custom IgBLAST database. This database is built from monoclonal antibody heavy and light chain reference sequences from B18-383 and HA mouse models ...

Methanol-fixed cells were then incubated with sera diluted 1:300. IgG AF488-antibody (Dianova, #109-545-003), commercial rabbit SYN1/SYN2 antibody (Synaptic Systems, #106002) and anti-rabbit IgG AF594 ...

The membranes were blocked in TBST containing 5% non-fat dried milk for 1h, then incubated with primary antibodies including p-STAT3 (Cell Signaling Technology, United States), STAT3 (Cell Signaling ...