To carry out real-time PCR, researchers have to choose not only what primers to design (see Box 2) but also what detection chemistry to use. In many cases these decisions will be influenced ...

Reliable PCR and qPCR results depend on more than just enzymes and primers - the quality of your consumables can make or break an assay. Azenta Life Sciences has developed its 4titude® range of ...

This manuscript characterizes a mutated clone of RNA polymerase I in yeast, referred to as SuperPol, to understand the mechanisms of RNA polymerase I elongation and termination. The authors present ...

In this valuable study, the authors demonstrate that TCF7L2 plays a role in the pathogenesis of cachexia in a mouse model of GI cancer. The results are solid, although future studies will need further ...

RT-nested-PCR assay using primers selected for matching high homology regions in the nucleocapsid protein in situ of ARAV is a rapid and efficient tool for diagnosis without the need for biosafety ...

coupled with different GM event specific primer-probe systems (two primers/one probe). These assembled real-time PCR methods are validated by the CRL and, when they are found to perform well ...

To train new people to work with California specialty crops and update the skills of current workers, educators from UC Davis ...

The actives are so fresh and potent, each bottle is designed to be used within nine weeks—an indulgent ritual in real time. Made in small ... when applying.) This primer gel delivers a beautiful ...

2019, but briefly: all qPCR assays were run on the 7500FAST Real-Time PCR Systems and either SYBR Fast or Taqman Fast Advanced mastermixes (Applied Biosystems, Waltham, MA, USA). All primers and ...

for reverse transcription and quantitative fluorescence PCR. For the quantitative polymerase chain reaction study, a CFX Connect Real-Time System (BIO-RAD) was used. Primers for TNF-α, IL-1β, and IL-6 ...