A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...

I identified and characterized the first plasmid from a Mycoplasma, and contributed to efforts in the laboratory to create complete restriction maps of Mycoplasma genomes. In my first post-doctoral ...

Restriction sites, like airline hubs, aren't always where you need them, and sometimes they aren't there at all. Recombinase-based methods allow scientists to move pieces of DNA from plasmid to ...