This valuable study focuses on defining how the HSP70 chaperone system utilizes J-domain proteins to regulate the heat shock response-associated transcription factor HSF1. Using a combination of ...

Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry, State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Center for New Organic ...

Structural studies of membrane proteins historically have relied almost exclusively on first removing membrane proteins from the membrane with dispersive agents like detergents. But dispersive agents ...

Protein concentration was determined using the BCA Assay Kit (Beyotime, China). Protein were separated by SDS-PAGE and then transferred to a PVDF membrane. The membrane was then blocked with 5% milk ...