The cost of making a Phycoerythrin conjugated tetramer is $800. The most expensive reagents are the biotin ligase (BirA from Avidity, LLC) and the PE-conjugated streptavidin (Prozyme). On occasion, it ...

An alternative approach to generate monovalent (strept)avidin is to titrate in three equivalents of biotin per tetramer, but this is unsatisfactory because it generates a statistical mixture of ...

Prepare PBMC, splenocytes, lymph node cells, cell line cells, etc. at a concentration of 2-5 x 107 cells per ml in your favorite FACS buffer (FB, usually PBS + 2% calf serum + 0.1% sodium azide). Add ...

Therefore, the failure of tetramer production happens occasionally. In that case, a second attempt for the MHC/peptide folding and chromatographic purification will be tried with no additional charge.

) are partnering to develop and commercialize renewable specialty chemicals, with some relevant findings already in renewable wax structures. Elevance (Lisle, IL) utilizes Nobel Prize winning olefin ...

The biotin-avidin system plays a major role in these applications as it exhibits highly specific and strong binding affinity. Another distinctive advantage of this system are the four identical ...