unr.edu5y
Sanger sequencing: Common primers provided by the Nevada Genomics Center
All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...
Nature4y
Schematic for adapter and primer design for the two rare cutters EcoRI and PstI and the frequent cutter MseI
Adapters consist of a core sequence (black) and an enzyme-specific sequence (red). The enzyme-specific sequence allows the ligation of the adapters to the resulting restriction fragments (green ...
unr.edu5y
Sanger sequencing: Optimal amount of template and primer
At the Nevada Genomics Center we offer DNA sequencing using dye-terminator Sanger sequencing with analysis on an Applied Biosystems SeqStudio Genetic Analyzer. A Sanger sequencing reaction is run with ...