Southern transfer and hybridization ... 7| Cut a piece of nylon or nitrocellulose membrane and two sheets of thick blotting paper ∼1 mm larger than the gel in each dimension.

The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...

Foundations | The Birth of the Southern Blot, 1975 Courtesy of Ed Southern Gels used ... carried through with the flow it would be possible to capture them on a nitrocellulose membrane, using the ...