To generate the hairpin primer, select a 'sense' sequence ... the template oligonucleotide by incorporating miR-30 microRNA (miRNA) sequences (as DNA) into the target sequence (see Fig.

so what is the advantage of the ac-pre-miRNA substrate? The authors suggest that cleavage of the 3′ arm of the hairpin might contribute to strand selection or that cleavage of the 3′ arm might ...