Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...
Here, the authors present a proteomic and phosphoproteomic analysis of the human cell cycle in nontransformed cells using deep quantitative mass spectrometry by isobaric labelling. Structural and ...
The ability to identify cell cycle position through DNA content analysis ... The spatial relationship between GFP-expressing mitochondria and nuclei labeled with Nuclear-ID Red DNA stain in ...
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